Of note, in our population most of this effect was driven by an increase in the late diastolic component of mitral annulus motion (Figure 5, top panels), with only a nonsignificant trend towards a reduction in the early diastolic component
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The captured antigen was incubated with biotin conjugated human anti-HIV-1 antibody at 37 C for 1 hr, followed by incubation with enzyme, streptavidin-peroxidase and tetramethylbenzidine substrate at 37 C and room temperature/dark, respectively, for 30 minutes each
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